{"id":888,"date":"2018-04-26T03:08:56","date_gmt":"2018-04-26T03:08:56","guid":{"rendered":"http:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/?p=888"},"modified":"2018-04-26T03:08:56","modified_gmt":"2018-04-26T03:08:56","slug":"bioluminescent-art-final-results","status":"publish","type":"post","link":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/2018\/04\/26\/bioluminescent-art-final-results\/","title":{"rendered":"Bioluminescent Art (Final Results)"},"content":{"rendered":"<p>Hello Guys!<\/p>\n<p>It has been quite a journey, with many obstacles and unforseen challenges, but I have produced glowing green &amp; red bacteria art in petri dishes.<\/p>\n<p>&nbsp;<\/p>\n<p>Follow this link to watch my art grow, to watch my art glow:<\/p>\n<div style=\"width: 525px;\" class=\"wp-video\"><!--[if lt IE 9]><script>document.createElement('video');<\/script><![endif]-->\n<video class=\"wp-video-shortcode\" id=\"video-888-1\" width=\"525\" height=\"295\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/media.giphy.com\/media\/pcKlVYCeVO5JlhPdth\/giphy.mp4?_=1\" \/><a href=\"https:\/\/media.giphy.com\/media\/pcKlVYCeVO5JlhPdth\/giphy.mp4\">https:\/\/media.giphy.com\/media\/pcKlVYCeVO5JlhPdth\/giphy.mp4<\/a><\/video><\/div>\n<p>&nbsp;<\/p>\n<p>Here is a wordless version:<\/p>\n<div style=\"width: 525px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-888-2\" width=\"525\" height=\"295\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/media.giphy.com\/media\/2doytXWvhfmBmqOWQY\/giphy.mp4?_=2\" \/><a href=\"https:\/\/media.giphy.com\/media\/2doytXWvhfmBmqOWQY\/giphy.mp4\">https:\/\/media.giphy.com\/media\/2doytXWvhfmBmqOWQY\/giphy.mp4<\/a><\/video><\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>How I did it:<\/p>\n<ol>\n<li>Receiving a generous donation of green &amp; red florescent bacteria from Professor Farny at the Goddard Bio Labs, I went to my home lab\u00a0 in Gateway Park. I took a small colony from each bacteria plate &amp; put it in liquid LB media. I then labelled my new paint &amp; set it to grow at 37C overnight.\n<p><figure id=\"attachment_900\" aria-describedby=\"caption-attachment-900\" style=\"width: 800px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-900\" src=\"http:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Liquid-Cultures-1-800x584.jpg\" alt=\"\" width=\"800\" height=\"584\" srcset=\"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Liquid-Cultures-1-800x584.jpg 800w, https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Liquid-Cultures-1-768x561.jpg 768w, https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Liquid-Cultures-1.jpg 989w\" sizes=\"auto, (max-width: 767px) 89vw, (max-width: 1000px) 54vw, (max-width: 1071px) 543px, 580px\" \/><figcaption id=\"caption-attachment-900\" class=\"wp-caption-text\">Liquid Bacterial Cultures<\/figcaption><\/figure><\/li>\n<li>The same day, I also mixed up &amp; poured agar plates to grow the bacteria on. This process took 2-3 hours, as I had to heat up the agar to sterilize &amp; gel it (autoclaved).<\/li>\n<li><\/li>\n<li>The next day, I took my fully grown paint, my plates, some sterilizing ethanol (70%), wipes, and a paintbrush to a quiet lab benchtop and set to work. I sterilized my paintbrush, dipped into my liquid bacteria cultures, and very carefully &#8216;painted&#8217; on the agar plate. The bacteria cultures dried on clear, so I had to line up my designs underneath each agar plate carefully.\n<p><figure id=\"attachment_894\" aria-describedby=\"caption-attachment-894\" style=\"width: 567px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-894\" src=\"http:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Painting-Plates-1.jpg\" alt=\"\" width=\"567\" height=\"753\" \/><figcaption id=\"caption-attachment-894\" class=\"wp-caption-text\">Plate Painting Setup<\/figcaption><\/figure><\/li>\n<li>I repeated this process, each day adding more bacteria to expand the image with the bacteria&#8217;s growth phase.\n<p><figure id=\"attachment_895\" aria-describedby=\"caption-attachment-895\" style=\"width: 595px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-895\" src=\"http:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Painting-Plates-2-595x800.jpg\" alt=\"\" width=\"595\" height=\"800\" srcset=\"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Painting-Plates-2-595x800.jpg 595w, https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Painting-Plates-2.jpg 601w\" sizes=\"auto, (max-width: 595px) 100vw, 595px\" \/><figcaption id=\"caption-attachment-895\" class=\"wp-caption-text\">Painting Plates Continues<\/figcaption><\/figure><\/li>\n<li>I would also package up my bacteria plates each day in a SAFE styrofoam box, and transport that to the Goddard Labs, which have a UV-Darkreader. This wonderful machine allows one to place multiple agar plates next to each other &amp; dose them with the correct wavelength of radiation to make the florescent bacterias really glow! Here is some of my agar art illuminated:\n<figure id=\"attachment_899\" aria-describedby=\"caption-attachment-899\" style=\"width: 800px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-899\" src=\"http:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Final-Viral-800x606.jpg\" alt=\"\" width=\"800\" height=\"606\" srcset=\"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Final-Viral-800x606.jpg 800w, https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Final-Viral-768x581.jpg 768w, https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Final-Viral.jpg 992w\" sizes=\"auto, (max-width: 767px) 89vw, (max-width: 1000px) 54vw, (max-width: 1071px) 543px, 580px\" \/><figcaption id=\"caption-attachment-899\" class=\"wp-caption-text\">Final Viral- The Infection has fully spread!<\/figcaption><\/figure>\n<p><figure id=\"attachment_898\" aria-describedby=\"caption-attachment-898\" style=\"width: 800px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-898\" src=\"http:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Final-Bird-800x646.jpg\" alt=\"\" width=\"800\" height=\"646\" srcset=\"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Final-Bird-800x646.jpg 800w, https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Final-Bird-768x620.jpg 768w, https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-content\/uploads\/sites\/5\/2018\/04\/Final-Bird.jpg 933w\" sizes=\"auto, (max-width: 767px) 89vw, (max-width: 1000px) 54vw, (max-width: 1071px) 543px, 580px\" \/><figcaption id=\"caption-attachment-898\" class=\"wp-caption-text\">Bacteria Bird\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0*<em>May also be a jellyfish when viewed upside down*<\/em><\/figcaption><\/figure><\/li>\n<li>Sometimes, if I was working on only part of an image, I would store the other plates into the fridge to avoid unwanted growth or death of my art project.<\/li>\n<li>Finally, on the last day of growth, I created a GIF to show the growth of my art at a much faster rate.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>In addition to the above art, I also created another GIF of Bacteria Bird Growing:<\/p>\n<div style=\"width: 525px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-888-3\" width=\"525\" height=\"295\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/media.giphy.com\/media\/EQuc5U8ss6Jr8LcI2v\/giphy.mp4?_=3\" \/><a href=\"https:\/\/media.giphy.com\/media\/EQuc5U8ss6Jr8LcI2v\/giphy.mp4\">https:\/\/media.giphy.com\/media\/EQuc5U8ss6Jr8LcI2v\/giphy.mp4<\/a><\/video><\/div>\n<p>&nbsp;<\/p>\n<p>Thank you all for making the class projects so diverse &amp; interesting!<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Hello Guys! It has been quite a journey, with many obstacles and unforseen challenges, but I have produced glowing green &amp; red bacteria art in petri dishes. &nbsp; Follow this link to watch my art grow, to watch my art glow: https:\/\/media.giphy.com\/media\/pcKlVYCeVO5JlhPdth\/giphy.mp4 &nbsp; Here is a wordless version: https:\/\/media.giphy.com\/media\/2doytXWvhfmBmqOWQY\/giphy.mp4 &nbsp; &nbsp; How I did it: &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/2018\/04\/26\/bioluminescent-art-final-results\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Bioluminescent Art (Final Results)&#8221;<\/span><\/a><\/p>\n","protected":false},"author":57,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"cybocfi_hide_featured_image":"","_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-888","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/888","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/users\/57"}],"replies":[{"embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/comments?post=888"}],"version-history":[{"count":2,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/888\/revisions"}],"predecessor-version":[{"id":903,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/888\/revisions\/903"}],"wp:attachment":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/media?parent=888"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/categories?post=888"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/tags?post=888"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}