{"id":702,"date":"2018-04-12T11:39:39","date_gmt":"2018-04-12T11:39:39","guid":{"rendered":"http:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/?p=702"},"modified":"2018-04-12T18:15:49","modified_gmt":"2018-04-12T18:15:49","slug":"the-yeast-are-not-working-but-the-bacteria-is","status":"publish","type":"post","link":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/2018\/04\/12\/the-yeast-are-not-working-but-the-bacteria-is\/","title":{"rendered":"The Yeast are Not Working!! (But the Bacteria are)"},"content":{"rendered":"

Hello all,<\/p>\n

This week I am going to review everything I failed at so far & give a progress update:<\/p>\n

The Yeast<\/strong><\/p>\n

Last Friday, I went into Professor Farny’s lab to expose my plates to UV light & make them glow.<\/p>\n

The yeast do not glow with the intensity that I was expecting:<\/p>\n

\"\"\"\"<\/p>\n

 <\/p>\n

Fortunately, Professor Farny gave me some of her GFP Bacteria stock-<\/p>\n

\"\"<\/p>\n

Microscope\/ Yeast Glow Activation<\/strong><\/p>\n

Also, the original microscope I was using for images was not working:<\/p>\n

\"\"<\/p>\n

I am now going to use the “DarkReader” UV panel to make my microbes glow:<\/p>\n

\"\"<\/p>\n

Yeast Photo-Kill Exposure<\/strong><\/p>\n

I really tested this one- I left the yeast under the UV light for a solid 12 hours-<\/p>\n

This is the result:<\/p>\n

\"\"<\/p>\n

No Shebang! Plate is completely covered in yeast!<\/p>\n

I am temped to try out my experiment tomorrow in bacteria; they are far easier to kill that yeast, so it might work.<\/p>\n

 <\/p>\n

Instead, I have recently taken to painting my new GFP bacteria on plates. I have a new theme to go along with the new microbe => Imagine if, after just the right dose of radiation, your bacteria mutated, became sentient, and started crawling out of the petri dish.<\/p>\n

\"\"
A simple Staphylococcus on a plate<\/figcaption><\/figure>\n

That turns into that:<\/p>\n

\"\"
Staphylococcus is now angry and ready to take a bite out of you (For your meddling experiments.)<\/figcaption><\/figure>\n

I would like to set up my camera and take shots of growth, but that would require me to be in the lab, taking a picture every 15-30 minutes, for a solid 12 hours. I am thinking that I will make several plates with different stages of the “transformation” taking place, and line them next to each other on the darkreader.<\/p>\n

(Concept Picture- I am receiving red glowing bacteria tomorrow from Professor Farny, and would like to use both colors in my art for contrast.)<\/p>\n

\"\"<\/p>\n

One of the plates I poured agar into ended up with some bubbles, so I decided to go with it:<\/p>\n

\"\"
Bubble-Eyeball Creature<\/figcaption><\/figure>\n

 <\/p>\n

UPDATE: Day 2 Growth:<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

Hello all, This week I am going to review everything I failed at so far & give a progress update: The Yeast Last Friday, I went into Professor Farny’s lab to expose my plates to UV light & make them glow. The yeast do not glow with the intensity that I was expecting:   Fortunately, … <\/p>\n

Continue reading “The Yeast are Not Working!! (But the Bacteria are)”<\/span><\/a><\/p>\n","protected":false},"author":57,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"cybocfi_hide_featured_image":"","_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-702","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/702","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/users\/57"}],"replies":[{"embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/comments?post=702"}],"version-history":[{"count":5,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/702\/revisions"}],"predecessor-version":[{"id":764,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/702\/revisions\/764"}],"wp:attachment":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/media?parent=702"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/categories?post=702"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/tags?post=702"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}