{"id":627,"date":"2018-04-05T13:51:01","date_gmt":"2018-04-05T13:51:01","guid":{"rendered":"http:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/?p=627"},"modified":"2018-04-05T17:40:35","modified_gmt":"2018-04-05T17:40:35","slug":"yeasty-light-art-prototype","status":"publish","type":"post","link":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/2018\/04\/05\/yeasty-light-art-prototype\/","title":{"rendered":"Yeasty Light Art Prototype"},"content":{"rendered":"

Hello all!<\/p>\n

This week, I was tasked with making a yeast light art prototype. I decided to explore the painting technique this week, as this is a more sure bet than the long exposure, photographic technique.<\/p>\n

Show and Tell<\/strong>–<\/p>\n

Because my project uses genetically modified GFP yeast, I cannot transport it to class. I am, however, meeting with Professor Farny tomorrow- she has a lab facility in Goddard that can handle my yeast plates. I may be able to get the class access to the Goddard Lab to see the final work.<\/p>\n

Instead of the show and tell, I documented my process & results.<\/p>\n

To make my GFP Yeast Prototype, I first grew green florescent yeast in a liquid medium.\u00a0 I also grew non-GFP yeast in another tube.<\/p>\n

\"\"<\/p>\n

After that, I selected a petri dish with yeast-agar inside of it, and pipetted both yeast solutions into the dish. I did so randomly, so the yeast could grow as they pleased. I mixed the yeast strains so that they could grow together and compete for space on the dish, naturally producing complex patterns in the process.<\/p>\n

This is the dish @ 0 hours:<\/p>\n

\"\"<\/p>\n

The dish @ 1 day:<\/p>\n

\"\"<\/p>\n

After the yeast grew, I contacted Professor Duffy & he let me use his microscope to take pictures of my plate.<\/p>\n

\"\"<\/p>\n

The microscope is fitted with different light filters, to allow visualization of GFP (green) , BFP (blue), and mCHERRY (red). I took my sample, placed it under the microscope, and turned on the GFP filter (UV\/395nm wavelength).<\/p>\n

This was the result:<\/p>\n

\"\"<\/p>\n

The glowing portion is a GFP cell cluster that grew.<\/p>\n

This picture was taken at 8X, so it is a portion of the plate. Also, the microscope camera was acting up, so I had to line my phone up to the ocular to take the picture,which is why the picture has a low resolution.<\/p>\n

Optimization:<\/p>\n

There are several optimizations I would like to do for my yeast art:<\/p>\n

    \n
  1. Find a microscope\/ system to allow imaging of the whole plate<\/li>\n
  2. Use paint brushes to make designs on Agar plates<\/li>\n
  3. Get a camera to take a picture of my plates every minute for 12 hours…<\/li>\n
  4. Use my new long exposure setup to make glowing yeast photography<\/li>\n<\/ol>\n

    As for (1), I have also contacted Professor Farny; I heard from Professor Duffy that her IGEM team makes bacteria art on agar plates as well, and has a setup that will allow for full plate illumination. I will be able to do this on Friday.<\/p>\n

    I am also going to try exposing my plates to simple LEDs of the correct wavelengths, to see if this is an effective means of art visualization. If that works, I may be able to bring my art to class.<\/p>\n

    \"\"<\/p>\n

    Simple LED setup I rigged with a basic stamp<\/p>\n

    *I will also purchase a UV (395nm) flashlight to visualize the GFP.<\/p>\n

     <\/p>\n

    \"\"<\/p>\n

    This is an image I took with my LED setup. I currently am waiting for a UV LED to get delivered to illuminate the GFP, but the effect produced with the backlit red LED is still\u00a0 visually interesting.<\/p>\n

     <\/p>\n

    I have also made progress with my UV long-exposure setup:<\/p>\n

    \"\"<\/p>\n

    This is a UV sterilizing light-bulb, placed in a simple desk lamp, underneath a protective box. Yesterday, when the parts came in, I made a plate of GFP yeast, and exposed it to the light for about 5 hours. I am currently awaiting results.<\/p>\n

     <\/p>\n

    Materials:<\/strong><\/p>\n

    I have acquired most of the materials I need:<\/p>\n

      \n
    1. GFP Microbes<\/li>\n
    2. Petri Dishes<\/li>\n
    3. Cell Feed\/Agar<\/li>\n
    4. Basic GFP Microscope<\/li>\n
    5. UV Lamp<\/li>\n
    6. Transparent Printing Sheets<\/li>\n
    7. Box<\/li>\n<\/ol>\n

      I still need these materials\/accesses:<\/p>\n

        \n
      1. LED Lights\/ Illumination Visual tool (I have ordered this)-\u00a0http:\/\/https:\/\/www.amazon.com\/Escolite-Flashlight-Ultraviolet-Blacklight-Detector\/dp\/B008133KB4\/ref=pd_lpo_vtph_468_bs_t_1?_encoding=UTF8&psc=1&refRID=3MW14DHDR5QQEC1GZ1SJ<\/a><\/li>\n
      2. Access to Professor Farny’s IGEM Lab (GFP Scopes\/ visualization tools & possibly GFP Bacteria)*I will be meeting with her tomorrow to gain access to her lab*.<\/li>\n<\/ol>\n

         <\/p>\n

        Milestones:<\/strong><\/p>\n

          \n
        1. Get access to Farny Lab in Goddard (4\/6\/2018)<\/li>\n
        2. Get a whole GFP glowing agar plate image (4\/12\/2018)<\/li>\n
        3. Get a Photo-image in GFP yeast or bacteria (4\/12\/2018)<\/li>\n
        4. Get a camera & Document the development of GFP painting\/ photo (4\/16\/2018)<\/li>\n
        5. Create a yeasty design, scale & edit in photoshop to fit the petri dish (4\/16\/2018)<\/li>\n
        6. Test & optimize the design (4\/16\/2018-4\/24\/2018)<\/li>\n
        7. Grow the design multiple times & record the growth w\/pictures (4\/24\/2018)<\/li>\n
        8. Optimize & create a stop-motion growth video or time-lapse growth series (End)<\/li>\n
        9. *Allow class access to the Goddard Lab to see the art (end)*\u00a0 \u00a0 \u00a0*If possible<\/em><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

          Hello all! This week, I was tasked with making a yeast light art prototype. I decided to explore the painting technique this week, as this is a more sure bet than the long exposure, photographic technique. Show and Tell– Because my project uses genetically modified GFP yeast, I cannot transport it to class. I am, … <\/p>\n

          Continue reading “Yeasty Light Art Prototype”<\/span><\/a><\/p>\n","protected":false},"author":57,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"link","meta":{"cybocfi_hide_featured_image":"","_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-627","post","type-post","status-publish","format-link","hentry","category-uncategorized","post_format-post-format-link"],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/627","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/users\/57"}],"replies":[{"embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/comments?post=627"}],"version-history":[{"count":9,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/627\/revisions"}],"predecessor-version":[{"id":667,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/627\/revisions\/667"}],"wp:attachment":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/media?parent=627"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/categories?post=627"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/tags?post=627"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}