{"id":391,"date":"2018-03-27T20:44:09","date_gmt":"2018-03-27T20:44:09","guid":{"rendered":"http:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/?p=391"},"modified":"2018-03-27T20:44:09","modified_gmt":"2018-03-27T20:44:09","slug":"glowing-yeast-light-art","status":"publish","type":"post","link":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/2018\/03\/27\/glowing-yeast-light-art\/","title":{"rendered":"Glowing Yeast Light Art"},"content":{"rendered":"

For my project, I will be focusing on using glowing florescent yeast and\u00a0 strains to make light art. I have access to Professor Young’s stocks of glowing microbes, so I will be making art, in petri dishes, at Gateway.<\/p>\n

Logistics<\/strong><\/p>\n

The microbes produce various strains of florescent proteins. These proteins need to first absorb light to gain the energy they need to emit light. For example, a green florescent protein, or GFP, needs to receive light at an approximately 383 nm wavelength, which is blue-ultraviolet light. In return, GFP will emit a strong green light, at about 509 nm. Because of this, I will need either the correct-colored LEDs, or white LEDs and the appropriate spectra filters to make my art visible.<\/p>\n

 <\/p>\n

I have a few ideas that I would like to try out to make light art:<\/p>\n

Painting<\/strong><\/p>\n

This is the least intensive method for making light art:<\/p>\n

    \n
  1. Culture the correct FP-coding cells<\/li>\n
  2. Draw the image on a petri dish (under sterile hood)<\/li>\n
  3. Wait (overnight) for the drawing to develop<\/li>\n<\/ol>\n

    I would like to see if I can make a time-lapse video of the microbe growth.<\/p>\n

    Here are some examples of microbe painting from Pinterest:<\/p>\n

    \"\"
    Big Bacteria at Labagawa<\/figcaption><\/figure>\n
    \"\"
    Light Tiger<\/figcaption><\/figure>\n

     <\/p>\n

    Photonegatives<\/strong><\/p>\n

    This technique requires a little bit more of a setup from painting.<\/p>\n

      \n
    1. Create an image in Photoshop<\/li>\n
    2. Print image on transparent printing sheet<\/li>\n
    3. Put image on the top of a cell culture dish<\/li>\n
    4. Seed agar plate with cells<\/li>\n
    5. Turn on UV radiation with the dish inside the hood.<\/li>\n
    6. Leave cells for 1 hr +<\/li>\n
    7. Remove and check the next day for the image<\/li>\n<\/ol>\n

      Result are visually stunning though! (This example is also from Pinterest):<\/p>\n

      \"\"
      Bacteriosaur!<\/figcaption><\/figure>\n

      I made a first-pass attempt at this kind of artwork using yeast cells in the lab last week- I definitely need to optimize the process.<\/p>\n

      \"\"
      X-Wing?<\/figcaption><\/figure>\n
      \"\"
      Noooooo<\/figcaption><\/figure>\n

      I believe this happened because yeast cells are much more robust than the bacteria cells that other artists have used. I am going to try this method with bacteria if I can- they die much easier with UV exposure.<\/p>\n

      I am also going to buy a UV sterilizing light & expose the yeast to it overnight to see if that works…<\/p>\n

      \"\"
      My Simple UV Box Setup- For Long Exposures<\/figcaption><\/figure>\n

      3D Printing & Sculpture<\/strong><\/p>\n

      If I have time, I would like to try to 3D print non-circular petri dishes to grow my cells in.\u00a0 In addition, I have an Idea to make a 3D petri dish lamp using an LED as a light source for the yeast grown in the petri dishes.<\/p>\n

      \"\"<\/p>\n

      (Glowing cell culture disco ball???)<\/p>\n

      The lamp will be a small and roughly circular ball formed from petri dishes attached together. In the middle will be a blue LED; this LED will be the proper wavelength to make the yeast in the ptri dishes glow green. As a result, the lamp will appear dimly yellow from the agar, with glowing green patches where the yeast are, and the natural blue from the LED will shine through the gaps in-between the petri dishes.<\/p>\n

      Please feel free to reach out to me if you have any suggestions or other ideas!<\/p>\n","protected":false},"excerpt":{"rendered":"

      For my project, I will be focusing on using glowing florescent yeast and\u00a0 strains to make light art. I have access to Professor Young’s stocks of glowing microbes, so I will be making art, in petri dishes, at Gateway. Logistics The microbes produce various strains of florescent proteins. These proteins need to first absorb light … <\/p>\n

      Continue reading “Glowing Yeast Light Art”<\/span><\/a><\/p>\n","protected":false},"author":57,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"cybocfi_hide_featured_image":"","_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-391","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/391","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/users\/57"}],"replies":[{"embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/comments?post=391"}],"version-history":[{"count":3,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/391\/revisions"}],"predecessor-version":[{"id":410,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/posts\/391\/revisions\/410"}],"wp:attachment":[{"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/media?parent=391"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/categories?post=391"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.joshuarosenstock.com\/teaching\/lightart-d18\/wp-json\/wp\/v2\/tags?post=391"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}